Bulletin of Rehabilitation MedicineBulletin of Rehabilitation Medicine2078-19622713-2625National Medical Research Center for Rehabilitation and Balneology59572910.38025/2078-1962-2023-22-2-42-51Research ArticleEffect of Bioplastic Material on Adhesion, Growth and Proliferative Activity of Human Fibroblasts when Incubated in Solutions Mimic the Acidity of Wound an Acute and Chronic InflammationMarkovPavel A.<p>Ph.D. (Biol.), Department of Biomedical Technologies</p>markovpa@nmicrk.ruhttps://orcid.org/0000-0002-4803-4803EreminPetr S.<p>Researcher, Laboratory of Cellular Technologies, Department of Biomedical Technologies</p>ereminps@gmail.comhttps://orcid.org/0000-0001-8832-8470PaderinNikita M.<p>Researcher, Department of Molecular Immunology and Biotechnology</p>paderin_nm@mail.ruhttps://orcid.org/0000-0001-5313-7105GilmutdinovaIlmira R.<p>Ph.D. (Med.), Head of the Department of Biomedical Technologies</p>gilm.ilmira@mail.ruhttps://orcid.org/0000-0001-6743-2615KostrominaElena Yu.<p>Ph.D. (Biol.), Senior Researcher, Department of Biomedical Technologies</p>bioimed07@hotmail.comhttps://orcid.org/0000-0002-9728-7938GrebenAnastasia I.<p>Junior Researcher, Department of Biomedical Technologies</p>aik-nastya@mail.ruhttps://orcid.org/0000-0002-2423-523XFesyunAnatoliy D.<p>Dr Sci. (Med.), Acting Director</p>nmicrk@nmicrk.ruhttps://orcid.org/0000-0003-3097-8889Medical Research Center for Rehabilitation and BalneologyInstitute of Physiology of Federal Research Centre «Komi Science Centre of the Urals Branch of the Russian Academy of Sciences»2409202322242512409202324092023Copyright © 2023, Markov P.A., Eremin P.S., Paderin N.M., Gilmutdinova I.R., Kostromina E.Y., Greben A.I., Fesyun A.D.2023<p><strong>INTRODUCTION.</strong> One of the key stages of wound healing is the phase of inflammation, which is a transitional process between hemostasis and wound healing. Each stage of the inflammatory-reparative process is characterized by its own value of the acidity of the wound bed. For example, in the acute stage of inflammation, the acidity of the medium in the wound bed decreases to a pH of 5.5-6. The chronic stage of the inflammatory process, on the contrary, is accompanied by an increase in pH to 8. To date, so far, the effect of biomaterials containing components of the intercellular matrix of the human dermis on fibroblasts under acidosis and alkalosis has not been fully investigated.</p> <p><strong>AIM. </strong>To define the effect of bioplastic material based on collagen, hyaluronic acid and elastin on the viability and proliferative activity of human fibroblasts in conditions simulating the acidity of acute and chronic wounds.</p> <p><strong>MATERIAL AND METHODS. </strong>Bioplastic material was made according to the method described in patent RU 2722744. Adhesive properties and proliferative activity of human fibroblasts were assessed visually using fluorescent microscopy. The number of apoptotic and dead cells was assessed by flow cytometry (BD FACSCanto II) using the commercial FITC Annexin V Apoptosis Detection Kit I (BD Pharmingen). The strength, Youngs modulus, and elasticity of the gels were determined on a TA.XT-plus texture analyzer (Stable Micro Systems, Great Britain).</p> <p><strong>RESULTS AND DISCUSSION. </strong>Using the methods of luminescent microscopy and flow cytometry, we found that the cell viability (namely, adhesive properties and proliferative activity) decreases after incubation on condition mimic of physiological acidosis. 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