Issue 1-83, 2018

Ozonotherapy as a Factor Having an Impact on the Results of Cell Therapy of Experimental Liver Cirrhosis

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1 Osipov B.B.

1 UO Gomelskij Gosudarstvennyi Medicinskij Universitet, Gomel, Belarus


ABSTRACT

The purpose of our study was to evaluate the impact of ozonotherapy on the results of cell therapy of experimental livercirrhosis. White Wistar male rats (n=45) were used as an object of our study. After modeling of liver cirrhosis a course ofozonotherapy for one group of rats (group 3) was performed. Than animals of groups 2 and 3 were treated with single intraportalinjection of autologous mesenchymal stem cells (MSCs). Animals had been sacrificed before cell therapy, 30 daysand 90 days after cell transplantation, pathohistological state of liver, prooxidant and antioxidant markers of blood serumhad been examined with further statistical analysis.Administration of autologous MSCs in experimental liver cirrhosis leads to statistically significant consistent reductionof thickness of connective-tissue septa in liver (р=0,0041), decreasing of prooxidant markers concentration and increasingof antioxidant markers activity (p=0,009) 30 days and 90 days after cell transplantation in comparison with control animals.Course of ozonotherapy before cell therapy leads to statistically significant improvement of mentioned indicators in comparisonto those in case of monotherapy with stem cells (р<0,05).Transplantation of autologous MSCs leads to consistent improvement of histological state in liver cirrhosis 30 days and90 days after cell therapy. Ozonotherapy facilitates the improvement of the results of cell therapy of experimental liver cirrhosis.The mechanism of the positive impact of ozonotherapy is probably in its antioxidant effect, which occur in statisticallysignificant decreasing of prooxidant markers concentration and increasing of antioxidant markers activity.


KEYWORDS: mesenchymal stem cells, liver cirrhosis, ozonotherapy, glutathione peroxidase, morphometry, cell therapy, antioxidants, prooxidant markers


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This is an open article under the CC BY 4.0 license. Published by the National Medical Research Center for Rehabilitation and Balneology.