Issue 20-2, 2021

Original article

https://doi.org/10.38025/2078-1962-2021-20-2-104-108

Prevention of Postradiational Disorders in the Testes of Rats with the use of Magnetic Field

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1 ORCIDYuri N. Korolev, 1 ORCIDLyudmila A. Nikulina, 1 ORCIDLyubov V. Mikhailik

1 National Medical Research Center of Rehabilitation and Balneology, Moscow, Russian Federation


ABSTRACT

The action effects of the low-intensity low-frequency magnetic field (LMF) are mainly studied in its therapeutic use and to a much lesser extent in the mode of primary prevention.

AIM. To identify adaptive metabolic and ultrastructural changes in the testes of rats under the preventive action of LMF under radiation conditions.

MATERIAL AND METHODS. The experiments were carried out on 28 mature non-linear male rats weighing 180-200 g. The animals were divided into 3 groups: in the experimental group, the animals received a course of LMF procedures followed by radiation exposure; in the control group, the animals were subjected only to radiation exposure; in the intact group, the animals were not exposed to any effects. The animals were slaughtered the day after the radiation exposure. The research methods were used: biochemical (to determine the content of RNA, DNA, antioxidant activity of the testes), light – optical (to count the number of convoluted seminiferous tubule – CST, exfoliated cells, spermatogonia, to determine the index of spermatogenesis); transmission electron microscopy; morphometric analysis of mitochondria (number, average and total area).

RESULTS. The preventive effect of LMF enhanced the adaptive capabilities of the body and increased the resistance of the testes to the effects of radiation. This was manifested in an increase in the power of the antioxidant system, activation of the cellular and intracellular regeneration processes, and a decrease in the permeability of the structures of the CST’s own shell.

CONCLUSION. The obtained data substantiate the possibility of using LMF as a means of protecting the organs of the reproductive system in the complex prevention of the body under the influence of radiation.



KEYWORDS: prevention, radiation, low-frequency magnetic field, rat testes, spermatogonia, Sertoli cells

FUNDING: The study had no sponsorship

CONFLICT OF INTEREST: The authors declare no apparent or potential conflicts of interest related to the publication of this article

FOR CITATION: Korolev Y.N., Nikulina L.A., Mikhailik L.V. Prevention of Postradiational Disorders in the Testes of Rats with the use of Magnetic Field. Bulletin of Rehabilitation Medicine. 2021; 20 (2): 104-108. https://doi.org/10.38025/2078-1962-2021-20-2-104-108

FOR CORRESPONDENCE:

Yuriy N. Korolev, e-mail: korolev.yur@yandex.ru


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This is an open article under the CC BY 4.0 license. Published by the National Medical Research Center for Rehabilitation and Balneology.