Issue 23-6, 2024

Original article

https://doi.org/10.38025/2078-1962-2024-23-6-45-53

Comparative Evaluation of the Biocompatibility of Sponges Based on Different Chitosan Salts in vitro

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1,2,* ORCIDMarina V. Volkova, 2 ORCIDYan B. Kovalevsky, 1 ORCID Petr S. Eremin, 3 ORCIDKonstantin N. Demchenko, 1 ORCID Ilmira R. Gilmutdinova, 1 ORCIDPavel A. Markov

1 National Medical Research Center for Rehabilitation and Balneology, Moscow, Russia
2 Chemical company “Orion” LTD, Saint-Petersburg, Russia
3S.M. Kirov Military Medical Academy, Saint-Petersburg, Russia


ABSTRACT

INTRODUCTION. The relevance of the study is due to the increase in the number of human skin lesions as a result of natural and man- made emergencies. Among the natural polymers widely used for the manufacture of wound dressings is chitosan, but its poor solubil- ity is a technological and biological limitation for its effective inclusion and use as a component of wound dressings. One way to solve the problem is to modify chitosan with glycolic, lactic or ascorbic acids. For eff ective and adequate use of chemical modifications of chitosan as a material for wound dressings, it is necessary to evaluate their biocompatibility.
AIM. In vitro assessment of the cytotoxicity and hemostatic properties of sponges based on chitosan modified with glycolic, lactic and ascorbic acids.
MATERIALS AND METHODS. Sponges based on water-soluble chitosan salts were provided by Chemical Company “Orion” LTD. The biocompatibility of the obtained products was assessed on the culture of mesenchymal stem cells, using light and fluorescent micros- copy. The metabolic activity of the cells (MTT-test) and the sorption properties of the biomaterials were assessed by spectrophotometry.
RESULTS AND DISCUSSION. Chitosan glycolate or chitosan lactate sponges ensure the survival of most adherent cells, while chitosan ascorbate sponges inhibit cell viability and growth. It was found that chitosan glycolate sponges have the highest blood sorption ca- pacity and are capable of absorbing over 700 μl of blood/cm3. Chitosan lactate and chitosan ascorbate sponges demonstrate a sorption capacity of 250 μl of blood/cm3. However, the ascorbate-based material inhibits the metabolic activity of cells.
CONCLUSION. Based on the research results, sponges made of chitosan glycolate or lactate, which have good biocompatibility in vitro, can be used for further development of wound-healing coatings. In addition, sponges made of chitosan glycolate can be used as highly effective hemostatic materials. Sponges made of chitosan ascorbate require further development and separate study.


KEYWORDS: chitosan glycolate, chitosan lactate, chitosan ascorbate, cytotoxicity, hemostatic properties

FUNDING: The authors declare no external funding in the conduct of the study.

CONFLICT OF INTEREST: The authors declare no apparent or potential conflicts of interest related to the publication of this article.

FOR CITATION:

va M.V., Kovalevsky Ya.B., Eremin P.S., Demchenko K.N., Gilmutdinova I.R., Markov P.A. Comparative Evaluation of the Biocompatibility of Sponges Based on Different Chitosan Salts in vitro. Bulletin of Rehabilitation Medicine. 2024; 23(6):45-53. https://doi.org/10.38025/2078-1962-2024-23-6-45-53 (In Russ.).

FOR CORRESPONDENCE:

Marina V. Volkova, biotech.volkova@list.ru


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This is an open article under the CC BY 4.0 license. Published by the National Medical Research Center for Rehabilitation and Balneology.